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KMID : 0359319910310030329
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Korean Journal of Veterinary Research
1991 Volume.31 No. 3 p.329 ~ p.335
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Radiation - induced DNA strand breaks in EL4 cells and mouse spleen lymphocytes
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김태환/Kim, Tae Hwan
김성호/정인용/진수일/류성렬/조철구/Kim, Sung Ho/Chung, In Yong/Chin, Soo Yil/Yoo, Seong Yul/Cho, Chul Koo
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Abstract
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The filter elution technique was used to assay ^(600Co γ ray-induced DNA strand breaks(SB) in EL4 mouse leukemia cell and mouse spleen, lymphocyte.
The lymphocytes were stimulated with lipopolysaccharzde (LPS, 20㎍/㎖) to label [³H]thymidine. EL4 cells and lymphocytes in suspension were exposed at 0℃ to 0Gy, 1Gy, 5Gy, l0Gy or 15Gy for DNA single strand breaks(SSB) assay and 0Gy, 25Gy, 50Gy, 75Gy or l00Gy for DNA double strand breaks(DSB) assay of ^(60)CO radiation and elution procedure was performed at pH12.1 and 9.6.
The number of DNA strand breaks increased with increasing doses of γ rays. The strand scission factor(SSF) was estimated in each experiment (eluted volume 21㎖). The slope of SSB EL4 cells was 0.01301±0.00096Gy^(-1) (n=5), the slope of SSB for lymphocytes was 0.01097±0.00091Gy^(-1) (n=5) and the slope of DSB for lymphocytes was 0.001707±0.0000573Gy^(-1) (n=5). Thus EL4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes (p<0.005). The ratio of slope of dose-response relationship (SSF versus dose) of lymphocytes DNA SSB as compared with the slope of DNA DSB was 6.4.
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